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TCMK-1 华体会体育电子 购买

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    ATCCCCL-139TCMK-1 华体会体育电子 购买

    基本信息
    资源编号 ATCCCCL-139
    资源名称 TCMK-1
    种属 Mus musculus, mousekidney
    类型 SV40 transformed
    形态 epithelial
    提供形式 frozen
    致病类型 normal
    安全等级 2
    培养方法
    培养基 The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
    传代方法 Volumes used in this protocol are for 75 cm2 flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes. Remove and discard culture medium. Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum which contains trypsin inhibitor. Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes). Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal. Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting. Add appropriate aliquots of the cell suspension to new culture vessels. Incubate cultures at 37°C.
    Subc*tion Ratio: A subc*tion ratio of 1:2 to 1:8 is recommended
    Medium Renewal: Every 2 to 3 days Note: For more information on enzymatic dissociation and subculturing of cell lines consult Chapter 10 in Culture of Animal Cells, a manual of Basic Technique by R. Ian Freshney, 3rd edition, published by Alan R. Liss, N.Y., 1994.
    生长条件 Temperature: 37°C
    生长特性 adherent
    存储条件 liquid nitrogen vapor phase;Freeze medium: Complete growth medium 95%; DMSO, 5%
    Storage temperature: liquid nitrogen vapor phase
    详细说明
    描述 About 2% of the cells are positive for SV40 viral antigen, but virus is not recoverable.
    Tested and found negative for ectromelia virus (mousepox).
    Age weanling
    Strain C3H/Mai
    Karyotype modal number = 78; range = 64 to 157.All cells showed 2-6 acrocentric chromosomes with secondary constrictions and 1-4 biarmed chromosomes per cells. Sixty percent of the cells had one or more minute chromosomes.
    Derivation The cells are SV40 transformed and are positive for SV40 T-antigen.
    Antigen Expression H-2k
    Tumorigenic No
    Effects No, in immunosuppressed mice
    Yes, in semisolid medium
    Virus Susceptibility Vesicular stomatitis, Glasgow (Indiana) Human poliovirus 1
    Virus Resistance poliovirus 1
    Year of Origin 1963

    ATCCCCL-139TCMK-1 华体会体育电子 购买

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